Comparison of artificial insemination methods in sheep using semen from Ovis
نویسندگان
چکیده
Reproductive biotechnologies find application both in conservation of endangered species and to improve production from domestic animals. The cross between Mouflon and domestic sheep could be exploited to produce suckling lamb, a foodstuff with high organoleptic qualities and that could meet modern consumer demand. Mouflon semen was collected from two adult males using an artificial vagina during the sexual season. 80 ewes were synchronized by insertion of intravaginal sponges (40 mg FGA), and by an intramuscular injection of 400 IU of PMSG at sponge removal. 40 ewes (Group A) were inseminated into the cervix using refrigerated semen (+4°C, 0.25 ml and 800 million spermatozoa per dose) 56 hours after the sponge removal. 40 ewes (Group B) were inseminated into the uterus using the laparoscopic technique 54 hours after sponge removal using cryopreserved and later thawed semen (0.25 ml and 400 million spermatozoa). Chi-squared test showed values significantly higher (P<0.01) in fertility rate in Group A (47.5% vs 32.5%). In both groups prolificity rate was similar (1.1%). In conclusion, intracervical method with the use of fresh semen, gives better reproductive performances, and it is easy to apply for obtaining cross lamb on a large scale. On the other hand, result regarding laparoscopic-intrauterine technique could be considered good and the method is very useful because it allows to use semen in a rational way and in a longer lapse of time.
منابع مشابه
Effect of time and depth of insemination on fertility of Bharat Merino sheep inseminated trans-cervical with frozen-thawed semen
BACKGROUND Artificial insemination (AI) can serve as a powerful tool to the sheep owners for making rapid genetic progress of their flock. The AI in sheep is mostly performed using fresh semen with two reasons i) lambing rate following trans-cervical AI with frozen semen is limited by the inability of frozen-thawed sperm to transit the cervix and ii) the need of circumventing the cervical barri...
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